This effect of ccl4 appears to be reversible and dosedependent. We have investigated the role of cytochrome p4502e1 cyp2e1 in this interaction. Three groups of 10 rats each were subjected to experimental study. Hederin on carbon tetrachlorideinduced hepatotoxicities were investigated in mice. In this study the protective effects of davallialactone 1, isolated from inonotus xeranticus, have been examined against carbon tetrachloride ccl4 induced acute liver injury. Antioxidant potential of xylopia aethiopica adewale o. Carbon tetrachloride ccl4 is one of the most dangerous hepatotoxic environmental pollutants thus this study aimed at investigating the potential preventive effect. This is mainly due to the effective antioxidant potential of nacetyl cysteine. The aim of this study was to establish the effect of. Susceptibility to chlordeconecarbon tetrachloride induced. It is also known that either ccl4 or sns can affect systemic inflammatory responses. The present study aimed at assessing the protective effect of indigofera oblongifolia on carbon tetrachloride ccl 4 induced hepatotoxicity. Carbon tetrachloride ccl4induced hepatic damage in. Riboflavin attenuates carbon tetrachlorideinduced oxidative stress.
The pathogenesis of the damage is multifactorial, ranging from oxidation, and in. The plant adhatoda vasica nees av of the acanthaceae. Alharbia, muzaffar iqbalb, sheikh fayaz ahmada a department of pharmacology and toxicology, college of pharmacy, king saud university, riyadh, saudi arabia. Mechanism of carbon tetrachlorideinduced hepatotoxicity. Carbon tetrachlorideinduced hepatotoxicity in rat is.
Protective effects of cholestin against carbon tetrachlorideinduced hepatotoxicity in rats. Sasa veitchii extract suppresses carbon tetrachloride induced hepato and nephrotoxicity in mice. Jun 15, 2018 despite being a cardinal experimental model, the induction of cirrhosis in rats by repeated exposure to carbon tetrachloride ccl4 has low reproducibility. Mixed halogenated methanes and ethanes, found as socalled disinfection byproducts at low concentration in drinking water, elicit symptoms of toxicity very similar to carbon tetrachloride, including carcinogenicity. Research article asian journal of biomedical and pharmaceutical sciences 1 3 2011, 2631 corresponding author. Journal of pharmacology and experimental therapeutics july 1964, 145 1 92101.
Glutathione plays an important role in the detoxification of most toxic agents. Hepatotoxicity and mechanism of action of haloalkanes. Hispidin analogue davallialactone attenuates carbon. In general, it is also already known that resistance to ccl4induced liver injury is influenced by the. The hepatoprotective effects of activaloe n931 complex, a mixture of aloe vera and silybum marianum, against acute and chronic carbon tetrachloride ccl4induced liver injuries were investigated. Ccl4induced liver damage was modeled in monolayer cultures of rat primary hepatocytes with a focus on involvement of covalent binding of cc14 metabolites to. Carbon tetrachloride an overview sciencedirect topics. Carbon tetrachlorideinduced hepatotoxicity in rat is reversed by treatment with ribo. If the inline pdf is not rendering correctly, you can download the pdf file here. Ccl4 is metabolized by cytochrome p450s cyp450s to ccl3, which is further converted into cl3coo, cl2c, and cocl2. Protective effect of secoisolariciresinol diglycoside in.
Carbon tetrachloride ccl4 is widely used as an animal model of hepatotoxicity and the mechanisms have been arduously studied, however, the contribution of the sympathetic nervous system sns in ccl4induced acute hepatotoxicity remains controversial. Carbon tetrachlorideinduced hepatotoxicity request pdf. Pdf hispidin analogue davallialactone attenuates carbon. Hederin prior to the administration of carbon tetrachloride significantly prevented the increase in serum alanine aminotransferase alt and lactate dehydrogenase ldh activity and lipid peroxidation in a dose dependent manner. Carbon tetrachloride induces hepatotoxicity via oxidative stress and inflammation. Posttranslational activation of endothelial nitric oxide. Nacetyl cysteine, hepatotoxicity, carbon tetrachloride carbon tetrachloride is commonly used as a model to evaluate hepatotoxicity 1.
Age is a risk factor for drug induced liver injury dili. In the present study, the hepatotoxicity induced by a single oral dose 2,000 mgkg of ccl 4 was compared between pregnant gestation days gd and 19 or postpartum postpartum days ppd 1, and 27 and nonpregnant rats. Hepatotoxicity of trichloroethylene carbon tetrachloride. O, onikanni a, sabiu saheed abstract the present study was carried out to evaluate the hepatoprotective effects of aqueous extract of. Comparison of vitamin e, lcarnitine and melatonin in ameliorating carbon tetrachloride and diabetes induced hepatic oxidative stress. Protective effects of silica hydride against carbon. Effect of three major nutrients on carbon tetrachlorideinduced. The present study was aimed to evaluate the efficacy of s. Carbon tetrachloride ccl 4 is widely used for experimental induction of liver damage5. Jul 24, 2018 in this work, various combinations of silymarin with natural bioenhancers such as lysergol l, piperine p and fulvic acid fa were prepared and their hepatoprotective activities were evaluated against carbon tetrachloride ccl4 induced hepatotoxicity in animal model.
In contrast to the early onset of carbon tetrachloride induced hepatotoxicity within 24 h of exposure, renal injury in humans and nonrodent species domestic cats and rabbits is typically encountered several days after carbon tetrachloride exposure guild et al. In this study the protective effects of davallialactone 1, isolated from inonotus xeranticus, have been examined against carbon tetrachloride ccl4induced acute liver injury. However, there is a limited understanding of pediatric dili. Consequently, the location of ccl4induced damage mirrors the oxygen gradient across the liver lobule. Trichloroethylene may also be received february 17, 1982. Puerarin, the main isoflavone glycoside found in the root ofpueraria lobata, has been used for various medicinal purposes in traditional chinese medicine for thousands of years. Hepatoprotective mechanisms of taxifolin on carbon tetrachloride. The present study was designed to evaluate the role of liv52 in carbon tetrachloride induced liver toxicity.
Carbon tetrachlorideinduced hepatotoxicity and nephrotoxicity in rats. Pdf protective effect of a mixture of aloe vera and silybum. Several studies have demonstrated that its toxicity is. Carbon tetrachloride ccl 4 induced hepatic damage in experimental sprague dawley rats. Yenhung yeha,b, youliang hsiehc, yating leed, chenghong. Carbon tetrachloride, when injected directly into the portal venous cannula of an isolated perfused rat liver, inhibited the net outward transport of hepatic triglycerides. Age is a risk factor for druginduced liver injury dili.
Acute hepatotoxicity was induced by intraperitoneal injection of ccl4 50 microlkg, and activaloe n931 complex at 85, 170, and 340 mgkg was administered orally 48, 24, and 2 h before and 6 h. Carbon tetrachloride hepatotoxicity pharmacological. Pdf standardization of carbon tetrachlorideinduced. Effects of yam peel extract against carbon tetrachloride. Consequently, the location of ccl4 induced damage mirrors the oxygen gradient across the liver lobule. Carbon tetrachloride ccl4 is widely used as an animal model of hepatotoxicity and the mechanisms have been arduously studied, however, the contribution of the sympathetic nervous system sns in ccl4 induced acute hepatotoxicity remains controversial. Standardization of carbon tetrachlorideinduced hepatotoxicity in the rat. Standardization of carbon tetrachloride induced hepatotoxicity in the rat. During the past 20 years, study of the fatty liver induced by carbon tetrachloride has passed through 2 periods of revolutionary change, and at the present time a third revolutionary change is taking place.
We studied the role and regulation of nos in carbon tetrachloride ccl4induced hepatotoxicity in newborn rats. Carbon tetrachloride ccl 4induced hepatic damage in experimental sprague dawley rats. Mechanism of carbon tetrachloride ccl4 hepatotoxicity. Hepatotoxins, such as ethanol, acetaminophen, and ccl 4, cause liver damage that is characterized by varying degrees of hepatocyte degeneration and cell death wu et al. Pdf solanum nigrum is used in hepatic disorders in folk medicines. Carbon tetrachloride cci4 was widely employed in human medicine as an. Carbon tetrachloride ccl 4 is well known to induce hepatotoxicity after being metabolized to trichloromethyl free radical ccl 3 by cyp2e1. The potent hepatotoxin carbon tetrachloride induced a 40fold elevation in dna replication 48 hr after a 400 mgkg dose, but no uds was observed at 2, 12, 24, or 48 hr posttreatment. Pdf carbon tetrachloride ccl4 induced hepatotoxicity in rats.
The present study aimed at assessing the protective effect of indigofera oblongifolia on carbon tetrachloride ccl 4induced hepatotoxicity. Thirty six rats of six per group were used in this study. Protective effect of indigofera oblongifolia in ccl4induced. Hepatotoxicity was induced in rats by carbon tetrachloride ccl 4 treatment. The potential protective effect of commelina nudiflora l. Xylopia aethiopica stem bark xasb on carbon tetrachloride ccl4 induced liver damage in sprague dawley rats. The prevention of carbon tetrachlorideinduced hepatotoxicity. Combination of metformin and luteolin synergistically. Ccl 4 induced liver damage was modeled in monolayer cultures of rat primary hepatocytes with a focus on involvement of covalent binding of ccl 4 metabolites to cell components andor peroxidative damage as the cause of injury. Comparison of two protocols of carbon tetrachlorideinduced.
Ccl 4 is a hepatotoxin widely used in animal models. Hepatocellular damage by reactive carbon tetrachloride metabolites. Pdf hepatoprotective effect of cleome gynandra on carbon. Weaning mice and adult mice exhibit differential carbon. Hispidin analogue davallialactone attenuates carbon tetrachlorideinduced hepatotoxicity in mice article pdf available in journal of natural products 7510 october 2012 with 79 reads. Here, 2weekold weaning and 8weekold adult male icr mice were intraperitoneally injected with ccl4 0. Hepatoprotective and antioxidant activity of bombax ceiba. Carbon tetrachloride is recognized as a major hepato toxin in humans 1. Amelioration of carbon tetrachlorideinduced hepatotoxicity. Carbon tetrachloride ccl4 induced hepatotoxicity in rats. Ccl 4 was once widely used as a dry cleaning solvent, precursor to refrigerant synthesis, propellant for aerosol cans, a fire extinguishing liquid, as a solvent for oils, fats, and. In the present study the hepatoprotective effect of methonolic extract of c.
Riboflavin reduces carbon tetrachlorideinduced increase in tnf riboflavin, a component of flavoproteins plays an important role in hepatoprotection. Pdf carbon tetrachloride ccl4 induced hepatotoxicity. We studied the role and regulation of nos in carbon tetrachloride ccl4 induced hepatotoxicity in newborn rats. Curative role of solanum nigrum article pdf available in journal of medicinal plant research 4. Ccl4induced liver injury led to significant increases in salt and sast activities, and these increases were limited by taxifolin and. Male swiss webster mice were administered retinol 75 mgkgd or vehicle for 3 days prior to ccl4 30 microlkg, ip. Protective effects of puerarin on carbon tetrachloride. In the mouse, retinol administration attenuates carbon tetrachloride ccl4 induced hepatic injury.
The principal findings are that the hepatotoxicity and mortality associated with cdccl4 interaction is highly agedependent for both sexes. It is a colourless liquid with a sweet smell that can be detected at low levels. The dominant notion guiding most of the thinking before 1948 was that toxic and nutritional fatty liver disease could be understood in terms of failure in transport of fatty acids as. There was marked hepatotoxicity occurring in both sexes as they reached 45 daysofage and females were considerably more susceptible than males to both cdccl4 induced hepatotoxicity and lethality. Carbon tetrachloride, also known by many other names such as tetrachloromethane, also recognised by the iupac, carbon tet in the cleaning industry, halon104 in firefighting, and refrigerant10 in hvacr is an organic compound with the chemical formula ccl 4. Carbon tetrachlorideinduced hepatotoxicity in pregnant and. Hepatotoxicity was induced in male wistar rats using ccl 4 1 mlday at an interval of 72 hours. Cleome gynandra has been used widely in indian indigenous system. The hepatoprotective effects of activaloe n931 complex, a mixture of aloe vera and silybum marianum, against acute and chronic carbon tetrachloride ccl4 induced liver injuries were investigated. Hepatotoxicity was assessed by estimating the activities of marker enzymes and by histological studies. Prevention of carbon tetrachloride induced hepatotoxicity in rats by. Crocin mitigates carbon tetrachlorideinduced liver toxicity in rats. The hypothesis that ccl4 poisoning exerts its hepatotoxic action via adrenalmedullary discharge of catecholamines and stimulation of sympathetic autonomic. Ccl 4induced liver damage was modeled in monolayer cultures of rat primary hepatocytes with a focus on involvement of covalent binding of ccl 4 metabolites to cell components andor peroxidative damage as the cause of injury.
Hepatoprotective effects of sea buckthorn hippophae. Pdf influence of liv52 on carbon tetrachloride induced. Protective effect of secoisolariciresinol diglycoside in carbon tetrachloride induced hepatotoxicity in rats sadiq s mareai 1, khalid m naji2, kavishankar gawli 3 and j rajesha 3 1department of chemistry, biochemistry division, university of thamar, thamar, yemen 2department of chemistry, university of sanaa, sanaa, yemen. Yuwen hsu, chiafang tsai, wenkang chen and fungjou lu, protective effects of seabuckthorn hippophae rhamnoides l. The purpose of this study was to investigate the protective effects of puerarin against hepatotoxicity induced by carbon tetrachloride cci4 and the mechanism of its hepatoprotective effect. Biochanin a protects against acute carbon tetrachloride. Role of cytochrome p4502e1 in retinols attenuation of carbon. Swertiamarin attenuates carbon tetrachloride ccl4induced liver. Resistance to carbon tetrachloride induced hepatotoxicity in mice which lack cyp2e1 expression. Protection from liver injury by 1 was confirmed by the observation of decreased serum transaminases and. The principle causes of carbon tetrachloride ccl 4 is induced hepatic damage in lipid peroxidation and decreased activities of antioxidant enzymes and generation of free. Mechanism of hepatotoxicity carbon tetrachloride ccl 4, a volatile inorganic compound, is a very welldescribed hepatotoxicant.